Biotechnology And Its Applications Chapter-Wise Test 1

Correct answer Carries: 4.

Wrong Answer Carries: -1.

Which of the following is incorrect about polymerase chain reaction (PCR)?

PCR amplifies specific DNA sequences.
PCR requires DNA primers for initiation.
PCR is used for synthesizing amino acids.
PCR is widely used in molecular diagnostics.
3

PCR is used for amplifying DNA sequences, not for synthesizing amino acids.

Which of the following is incorrect about Golden Rice?

Golden Rice is genetically modified to produce beta-carotene.
Golden Rice helps combat vitamin A deficiency.
Golden Rice increases soil nitrogen content.
Golden Rice was developed using genetic engineering.
3

Golden Rice is genetically modified to produce beta-carotene, but it does not increase soil nitrogen content.

Which technique is used in gene therapy to treat ADA deficiency?

RNA interference
Gene insertion using retrovirus
CRISPR-Cas9
Somatic hybridization
2

Gene therapy for ADA deficiency involves inserting a functional ADA gene using a retroviral vector.

Which of the following is correct about restriction endonucleases?

Restriction endonucleases join DNA fragments.
Restriction endonucleases synthesize RNA.
Restriction endonucleases cut DNA at specific sequences.
Restriction endonucleases repair damaged DNA.
3

Restriction endonucleases are enzymes that cut DNA at specific recognition sequences, essential in genetic engineering.

Which technique is used to deliver foreign genes into animal cells using fine glass needles?

Electroporation
Microinjection
Lipofection
Gene gun
2

Microinjection involves delivering foreign genes into animal cells using fine glass needles under a microscope.

Which of the following is incorrect about PCR?

PCR amplifies specific DNA sequences.
PCR requires DNA polymerase enzyme.
PCR is used to detect proteins in biological samples.
PCR involves denaturation, annealing, and extension steps.
3

PCR is used to amplify DNA, not to detect proteins; ELISA is used for protein detection.

Which enzyme helps in the formation of phosphodiester bonds between DNA fragments?

DNA polymerase
Ligase
Helicase
Endonuclease
2

DNA ligase helps in forming phosphodiester bonds between DNA fragments during recombinant DNA formation.

What is the main advantage of using genetically modified crops?

Increased pesticide use
Improved tolerance to abiotic stresses
Decreased photosynthetic efficiency
Reduced crop yields
2

GM crops show improved tolerance to abiotic stresses such as drought, heat, and salinity.

What is the term for the unauthorized use of biological resources without proper compensation?

Biopiracy
Bioethics
Bioremediation
Biosafety
1

Biopiracy refers to exploiting biological resources without authorization and fair compensation.

Which organism is genetically engineered to produce α-1-antitrypsin for treating emphysema?

Transgenic cow
Transgenic sheep
Transgenic mice
Transgenic bacteria
2

Transgenic sheep are genetically engineered to produce α-1-antitrypsin used in treating emphysema.

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